Age-Dependent Changes in Glutathione-S-Transferase: Correlation with Total Plasma Antioxidant Potential and Red Cell Intracellular Glutathione     

Maurya PK  Rizvi SI 《Indian journal of clinical biochemistry : IJCB》2010,25(4):398-400

The correlation between antioxidant capacity and oxidative damage during aging has been reported in several tissues in different species. Glutathione-S-transferases (GST) can metabolise endogenous and exogenous toxins and carcinogens by catalysing the conjugation of diverse electrophiles with reduced glutathione (GSH). We observe a significant (P < 0.001) increase in plasma GST activity as a function of human age (r = 0.5675). A significant (P < 0.001) positive correlation (r = 0.8979) is observed between GST activity and total plasma antioxidant potential measured as ferric reducing ability of the plasma (FRAP). GST activity and red cell intracellular GSH also show a significant positive correlation (r = 0.7014). We hypothesize that the increased activity of plasma GST is a manifestation of increased generation of ROS and a concomitant decrease in the level of plasma antioxidant capacity during aging.  相似文献   

成层地基固结性状中不同定义平均固结度研究分析   总被引：1，自引：0，他引：1  

罗勇  龚晓南  连峰 《科技通报》2006,22(6):813-816

一维地基固结理论从传统的太沙基一维固结理论到成层地基一维固结理论中。由于实际地基大多由性质不一的成状土组成,成层地基固结度计算一直为人们所关注。固结度有按沉降(压缩量)定义的地基总平均固结度和按作用的平均有效应力(或所消散的平均孔压)定义(简称按平均孔压定义)的地基总平均固结度。但对实际工程对它们的大小比较讨论甚少,对实际的选取应用没有理论上的支持。本文从一般均质地基的固结度定义入手,在相对比较理想的土体条件假设下,系统的比较了它们的大小区别,并在单面排水条件下结论Us>Up是一定成立的,并且根据以有的半理论解编制了相应的计算程序来说明本文的正确性。  相似文献   

Oxidative stress, α-tocopherol, ascorbic acid and reduced glutathione status in schizophrenics     

Gora Dadheech  Sandhya Mishra  Shiv Gautam  Praveen Sharma 《Indian journal of clinical biochemistry : IJCB》2006,21(2):34-38

A disturbance in the antioxidant defense system including α-tocopherol, ascorbic acid and reduced glutahtione metabolism due to free radical induced oxidative injury has been implicated in various neuro-psychiatric disorders. The roles of these antioxidants, changes in their blood levels and correlation with oxidative stress were studied in a common psychiatric illness Schizophrenia. Fifty-eight subjects of either sex ranging in age from 18–60 years divided into two age groups (≤40 and >40 years) diagnosed for schizophrenia, and forty age and sex-matched normal subjects as controls were included in the study. Blood samples were analyzed for malondialdehyde (MDA), α-tocopherol, total ascorbic acid (TAA), dehydro ascorbic acid (DHAA), reduced ascorbic acid (RAA), leucocyte ascorbic acid (LAA) and reduced glutathione (GSH). A decrease in the levels of α-tocopherol, total ascorbic acid and reduced glutathione was found in schizophrenics compared to normal controls. Further a significant rise in oxidative stress and decreased antioxidant status was observed in the chronic stage of schizophrenia as compared to those in acute condition. A significant rise in dehydroascorbic acid with concomitant fall in reduced ascorbic acid suggests scavenging action of ascorbic acid and its utilization with increased oxidative stress as indicated by high blood malondialdehyde levels. Leucocyte ascorbic acid, a better index of ascorbic acid status was also found to be reduced in schizophrenics, suggesting depletion of body stores of ascorbic acid and the condition worsened with advancing age.  相似文献   

Antioxidant status and peroxidative stress in mother and newborn —A pilot study     

Chitra Upadhyaya  Sandhya Mishra  P. P. Singh  Praveen Sharma 《Indian journal of clinical biochemistry : IJCB》2005,20(1):30-34

Serum antioxidant status and peroxidative stress was estimated in 30 pregnant women, their newborn infants and 25 non-pregnant women. Serum Ascorbic acid, α-tocopherol, retinol & β-carotene levels were found to be significantly reduced and peroxidative stress was significantly higher in mothers as compared to matched non-pregnant women. Newborn had significantly higher levels of ascorbic acid as compared to their mother but had significantly lower levels of lipid soluble vitamins. The peroxidative stress in newborn was found to be significantly less as compared to their mothers. A positive correlation of ascorbic acid and α-tocopherol levels between mother and newborn reflects that their status in mother does influence the newborn status.  相似文献   

Role of Oxidative Stress in Various Stages of Psoriasis     

Kadam DP  Suryakar AN  Ankush RD  Kadam CY  Deshpande KH 《Indian journal of clinical biochemistry : IJCB》2010,25(4):388-392

Psoriasis is a chronic inflammatory, proliferative skin disease characterized by pathological skin lesions due to various exogenous and endogenous factors. It is associated with a number of biochemical and immunological disturbances. Recently, it has been suggested that increased reactive oxygen species (ROS) production and compromised function of antioxidant system may be involved in the pathogenesis of this disease. In the present study, 90 psoriasis patients were selected. Disease severity was assessed by psoriasis area severity index score and grouped as mild, moderate and severe (each group consists of 30 subjects) and compared with 30 healthy controls. Serum levels of malondialdehyde, nitric oxide end products and the activities of antioxidant enzymes such as erythrocyte-superoxide dismutase, catalase and total antioxidant status were investigated in these groups/subjects. As compared to controls, we found severitywise significantly increased serum malondialdehyde, nitric oxide end products with decrease in erythrocyte-superoxide dismutase activity, catalase activity and total antioxidant status in patients with psoriasis suggesting worsening of the disease. It seems to be linked with the enhancement of Reactive Oxygen Species production and decreased antioxidant potential in psoriasis.  相似文献   

挑战性-阻碍性压力对员工创造力的影响研究          下载免费PDF全文

张亚军  肖小虹 《科研管理》2016,37(6):10-18

以压力二维结构为基础,探讨了挑战性压力和阻碍性压力对员工创造力的影响机制,重点分析创造力自我效能感的中介作用以及上下级关系的调节作用。选择企业员工及其直接上级作为研究对象,采用层级回归等方法分析336套配对数据。研究结果表明:挑战性压力正向影响员工创造力,而阻碍性压力负向影响员工创造力;创造力自我效能感在挑战性压力和阻碍性压力与员工创造力之间起到部分中介作用;上下级关系负向调节挑战性压力与员工创造力之间的关系,同时也正向调节阻碍性压力与员工创造力之间的关系。  相似文献   

Lipid peroxide levels and antioxidant status in alcoholic liver disease     

Seema Gupta  Rajesh Pandey  Ranjan Katyal  H. K. Aggarwal  R. P. Aggarwal  S. K. Aggarwal 《Indian journal of clinical biochemistry : IJCB》2005,20(1):67-71

The present study was conducted to evaluate some of the components of antioxidant defense system and oxidative damage in 20 male patients of alcoholic liver disease (ALD). The results were compared with 20 healthy male smokers and 20 healthy male non-smokers volunteers. Patients were subjected to detailed clinical examination and laboratory investigations. Blood samples were collected for estimating reduced glutathione (GSH), total thiols (T-SH) malondialdehyde (MDA), transaminases (AST, ALT), glutathione-S-transferease (GST) and gammaglutamyl transferase (GGT). Serum aspartate amino transferase (AST)/alanine amino transferase (ALT) ratio was significantly (p<0.01) reduced in ALD patients as compared to the controls. However, the core of utility of MDA and GST was found to be significantly (p<0.01) increased in ALD patients compared to controls. There was a significant negative correlation of MDA with both GSH and TSH. Plasma GGT levels were significantly (p<0.01) increased in alcoholics and the enzyme showed a significant positive correlation with MDA. These results give enough evidence of increased oxidative stress and compromised antioxidant defense system in patients with ALD.  相似文献   

Myeloperoxidase in Chronic Kidney Disease     

Madhusudhana Rao A  Anand U  Anand CV 《Indian journal of clinical biochemistry : IJCB》2011,26(1):28-31

Numerous lines of evidence implicate a role of myeloperoxidase (MPO) in the pathogenesis of cardiovascular disease (CVD). It is a well accepted fact that patients with chronic kidney disease (CKD) are at an increased risk for CVD. MPO is a pro-oxidant enzyme which could be involved in the increased susceptibility of these patients to CVD. Hence, the levels of plasma MPO was determined in healthy controls as well as in patients with CKD [stratified with the level of their kidney failure as CKD stages II–V (end stage renal disease)]. Plasma MPO was assayed by a spectrophotometric method. Serum urea and creatinine were estimated on a clinical chemistry analyzer using standard laboratory procedures. The mean plasma MPO levels were significantly lower with advancing stages of renal failure (P < 0.001). There was a positive correlation between MPO and GFR (r = +0.89, P < 0.001) and a negative correlation with urea (r = −0.85, P < 0.001) and creatinine (r = −0.82, P < 0.001). While an inverse association was observed between plasma MPO and urea in CKD patients, such an association was not observed in control subjects (P = 0.43). In conclusion, the decline in plasma MPO levels may be due to the inhibitory effect of uraemic toxins on the enzyme.  相似文献   

Evaluation of antioxidant properties of berries     

P. Rani  K. Meena Unni  J. Karthikeyan 《Indian journal of clinical biochemistry : IJCB》2004,19(2):103-110

The present paper focuses on assessing the levels of various enzymatic and non-enzymatic antioxidants in selected berries of Indian sub-continent viz., gooseberry, grapes, orange and tomato. This study has revealed that orange, tomato and grapes possess predominant quantities of enzymatic antioxidants namely SOD, catalase and glutathione peroxidase respectively. The levels of antioxidants analyzed namely reduced glutathione, vitamin C and vitamin A were maximal in grapes, orange and tomato respectively. All the extracts of berries included in the present study inhibited iron inducedin vitro lipid peroxidation in erythrocytes, the extend of inhibition being highest for gooseberry and orange compared to tomato and grapes which could be attributed to their in-built antioxidant system.  相似文献   

Risk factors for coronary heart disease in type II diabetes mellitus     

H. Surekha Rani  G. Madhavi  V. Ramachandra Rao  B. K. Sahay  A. Jyothy 《Indian journal of clinical biochemistry : IJCB》2005,20(2):75-80

Cardiovascular complications are the major cause of morbidity and mortality in diabetic patients. An attempt has been made to evaluate the risk factors for coronary heart disease in type II diabetics. In the present study the levels of fasting and postprandial plasma glucose, total cholesterol, low density lipoproteins, triglycerides were high and the levels of high density lipoproteins were low in the type II diabetics compared to controls. The markers of free radical induced injury i.e. malondialdehyde and nitrite/nitrate were high while total antioxidant status a marker for antioxidant protection against reactive oxygen species was low in diabetics compared to controls. The study therefore suggests the importance of assessing these markers of oxidative stress and antioxidant capacity along with the other routine investigations in diabetic patients for initiating antioxidant therapy in addition to primary and secondary preventive measures to mitigate the devastating consequences of diabetes leading to coronary heart disease.  相似文献